光子学报 ›› 2002, Vol. 31 ›› Issue (3): 277-280.

• 光生物学 • 上一篇    下一篇

He-Ne激光诱变选育高产白藜芦醇细胞系

郭斌, 尉亚辉, 曹炜   

  1. 西北大学生命科学学院, 西安, 710069
  • 收稿日期:2000-06-28 出版日期:2002-03-25 发布日期:2002-03-25
  • 通讯作者: 尉亚辉
  • 基金资助:
    陕西省教育厅基金资助项目

BREEDING VARIETY CELLS OF RESCERATROL BY He-Ne LASER INDUCED

Guo Bin, Wei Yahui, Cao Wei   

  1. Department of Biology, Northwest University, Xi’an 710069, China
  • Received:2000-06-28 Online:2002-03-25 Published:2002-03-25

摘要: 以葡萄皮脱分化组织为最初材料,用根癌农杆菌702菌株感染,通过纸电泳证明胭脂碱合成醇基因的存在,并在无激素培养基上培养5~6代以上,将此组织(MS)作为本次实验的研究对象.用He-Ne激光辐照,对MS进行诱变处理,通过继代选育,得到优良细胞系T12,其特点是:生长迅速,生长周期比对照组(CK)缩短10~15天;生长曲线的稳定期明显延长,白藜芦醇平均产量比MS组提高40%.通过稳定性实验证明T12组遗传性状是可靠的.

关键词: 激光, 诱变, 白藜芦醇

Abstract: Resveratrol is a phytoalexin in many food products,especially in grape,it was found to act as an antioxidant and has cancer chemopreventive activity in this paper,the effect of laser irradiation on grape peel callus which was infected by Agrobacterium tumefaciens 702 strain and was subcultured for 5-6 generation was observed.From the experiment,the biomass and content of resveratrol are greatly influenced by different induction time.A variety cells of resveratrol were bred-the yield of resveratrol were raised,the culture period was shorten from 35 days to 30 days,the biomass were raised,All characteristics of calle induced were steady.

Key words: Laser, Induction, Resveratrol